Starting material is immune cells. The receptor of interest is located in mitochondrial membrane. We are using radiolabeled ligand for this protein so we want to avoid or at least minimize protein denaturation.
Charles R Robertson
Not sure if you have seen this, but there is a Nature Protocol for isolating mitochondria-associated membrane proteins. Hope it helps.
http://www.nature.com/nprot/journal/v4/n11/full/nprot.2009.151.html
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