How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Can anyone explain how to calculate BRI (Biomarker response index)?

Hello everyone, Can anyone tell me how to calculate biomarker response index (BRI)? Suppose I have three treatment sets and one control set of plants. I have considered many cytotoxicity,...

28 October 2020 9,304 3 View

Is there any evidence that paracetamol or ibuprofen can be used to prevent COVID-19?

People are told that paracetamol or ibuprofen can only be used to treat the symptoms of COVID-19, but we think that taking paracetamol and vitamin C or ibuprofen and vitamin C every day is a...

01 July 2020 3,058 4 View

I want tips to ensure a successful experience in Allium cepa assay can you help me please?

I did the Allium cepa assay but didn't see any chromosomal abnormalities or divisions, I want tips to ensure a successful experience and What is the best dye? Acetocarmine or Basic fuchsine?

09 October 2019 9,914 3 View

What are the basis to accept one or another paramater of genotoxic activity (such tail length, tail moment or tail intensity) in the comet assay?

I have understood that analyzing genotoxicity with the comet assay depends on which parameter I decide to use, and according to some other publications & scientists, some of them prefer one...

06 July 2019 3,416 4 View

Suggest a good method to study chromosomal aberrations in plants how to prepare its slides for microscopic observations?

to study effects of silver nano particles on plant Genotoxicity

12 June 2019 141 1 View

Suggest a good method to study chromosomal aberrations in plants how to prepare its slides for microscopic observations?

to study effects of silver nano particles on plant Genotoxicity

05 June 2019 1,468 1 View

Detailed protocol to perform Comet assay?

Hi Everybody, I would like to perform Comet assay to test if a compound could be genotoxic on an adherhent cell line. Does anyone have experience abut it? Could you suggest me a detailed protocol...

05 June 2019 3,610 3 View

How to obtain a good spread in chromosomal abberration assay?

We are assessing the genotoxicity of my nanoparticles using chromosomal aberration assay in Swiss albino mice. We have tried optimizing the procedure using i.p. administration of colchicine at...

11 December 2018 9,509 14 View

Time periods for understanding Cytotoxicity and Genotoxicity?

Researchers incubate human cellines with their therapeutic compound for 3, 6, 12, 24 ans so on. Ideally, which assay can be done at specific time point to asses cytotoxicity and genotoxicity?

10 November 2018 5,850 0 View

why we use DMSO as solvent to assess cellular toxicity and do not use any other solvent in the assessment?

why we use DMSO as solvent to assess cellular toxicity and do not use any other solvent in the assessment?

10 November 2018 8,543 3 View