What is the MW of gp41, the replicative helicase from T4 phage?

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If my same paper published by two different journal without taking my consent what to do?

I have given one of my papers to publish in a journal. That was published and after the publication of that article, they again submit that article to a different publication without taking my...

13 June 2023 9,277 7 View

How can i restore my previous uploded research article, by mistake that was deleted?

By mistake, my previously uploaded article was deleted and my research interest score also became deleted with that deleted one. Anyone, please guide me on how could i restore the previous one.

03 June 2023 3,759 0 View

NetCDF files having the extension .nc4, into CSV file or geotiff file?

My goal is to prepare a script in python or R to work with netCDF files having the extension .nc4, example Daily GPM (Global Precipitation Measurement) precipitation data (Late Run) into CSV or...

08 April 2023 7,659 2 View

Is Item Analysis required during tool developmental, if items measures nominal value?

For example, I prefer to study in (A) Morning (B) Evening (C) Late Night

31 July 2022 6,457 5 View

Is it possible to obtain constant voltage in cuk converter for varying irradiation and temperature in a solar PV system with a resistive load ?

Actually I want to know the concept behind it, How it is possible. I am working on MPPT using Cuk converter.

28 July 2021 6,155 5 View

Can anyone give updated examples of AI tools or methods in Healthcare( E-Health)?

Updated very Recent Examples of AI tools in Healthcare

26 July 2021 873 5 View

Can I use some categorical variables as covariate while doing ANCOVA?

There are 4 continuous DV, some categorical variables like gender ( boys and girls), educational status of father (3 levels), educational status of mother (three levels), income ( three levels). I...

28 April 2021 567 8 View

I want some expert (free) advice and guidance in interpreting depositional environment from well logs in a deltaic setting. Anyone ready to help?

I am looking into the geophysical well logs from a coaliferous basin in India. I need some help in depicting the depositional environment from well logs.

02 February 2021 6,436 3 View

In Quantum Espresso, what is more appropriate choice for cell_dofree ('ibrav' or '2Dxy') in case of 2D materials such as MoS2 unit cell ?

While trying to do 'vc-relax' using pw.x in Quantum Espresso for MoS2 unit cell, I have developed a confusion regarding the selection of the cell parameters to be moved in cell_dofree card. As...

17 July 2020 4,276 3 View

How to find section moment ,shear force , rotation for a solid element C3D8R and T3D2 truss element as SF in outfield doesn't get analyse in Abaqus?

I'm trying to model a beam-column joint in Abaqus as C3D8R as solid element for concrete and T3D2 element as rebar . Please tell me how to get these parameters ( section moment , section shear...

01 August 2019 7,434 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View