I want some expert (free) advice and guidance in interpreting depositional environment from well logs in a deltaic setting. Anyone ready to help?

I am looking into the geophysical well logs from a coaliferous basin in India. I need some help in depicting the depositional environment from well logs.

02 February 2021 6,052 3 View

How to find section moment ,shear force , rotation for a solid element C3D8R and T3D2 truss element as SF in outfield doesn't get analyse in Abaqus?

I'm trying to model a beam-column joint in Abaqus as C3D8R as solid element for concrete and T3D2 element as rebar . Please tell me how to get these parameters ( section moment , section shear...

01 August 2019 7,225 3 View

Can anyone provide me experimental valid data of M30 concrete for concrete CDP with dt & dc value for Abaqus ?

I needed a M30 concrete data of inelastic strain vs compressive strength & dc and cracking strain vs tensile strength & dt experimental data for Abaqus .

28 June 2019 6,145 7 View

Is there any available software to design a control system in easy way?

Recently I have designed a automated gas leakage detection system using IoT. Now I want to analysis it further. What is the process to design/summarize it's control system.

16 July 2018 1,775 32 View

How can I get conductivity from the impedance analyzer (AC). I got resistance from the machine reading. How to convert it to conductivity?

Is it possible to measure directly the conductivity from the impedance analyzer?

24 February 2018 6,306 2 View

How can we reuse the wastes from Nuclear Power Plants? Specially when the plant is settled in a 3rd world country?

Major problem is , for a poor country there are lack opportunities to reuse the wastes, so I want develop a feasible waste management and recycling process specially applicable for the 3rd world...

07 November 2017 1,003 3 View

Is it possible to get the grain size of a continuous thin film (not assembly of nanoparticles) using Scherrer equation from XRD data?

I know that it is possible to get the mean particle size (crystallites) of nanoparticle assemblies using Scherrer equation. However, for continuous this films, does the same formula work to obtain...

20 July 2016 4,794 3 View

If Rapid quench flow is used and the product is freezed by plunge freezing tech, then how can that frozen sample be used as sample for Cryo EM?

Suppose I'm using a rapid quench flow instrument and stop the reaction at the desired time. The reaction is between helicase and DNA. I have provided all necessary conditions for the reaction to...

29 March 2016 9,153 1 View

At what pH I can cross link the substituted Cys residues in a subunit of the enzyme FoF1 ATP Synthase?

I have a double mutant aG218C/aI248C of the enzyme FoF1 ATP Synthase. Now I am thinking of cross linking these two substituted Cys residues in the a-subunit of the enzyme. At what pH I should do...

12 July 2015 2,489 2 View

How to get rid of pseudogene amplification?

I am working for a snake genus and I amplified multiple samples of the same species with a set of ND4 primers used in other 90% of the studies on snakes. However, when I cross checked for...

08 January 2014 678 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

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28 February 2021 9,936 2 View