Tanima Mallik

7 Questions 9 Answers 0 Followers

Questions related from Tanima Mallik

What is the MW of gp41, the replicative helicase from T4 phage?

What is the MW of gp41, the replicative helicase from T4 phage? What are the MW of other proteins of T4 phage replisome complex?

10 April 2016 3,669 2 View

At what resolution of Cryo EM we can see the quartanery structure of proteins?

What is near atomic resolution? In order to observe the quartanery structure of proteins or to observe the interaction between different proteins in a complex, what should be the resolution in...

04 April 2016 9,553 3 View

If Rapid quench flow is used and the product is freezed by plunge freezing tech, then how can that frozen sample be used as sample for Cryo EM?

Suppose I'm using a rapid quench flow instrument and stop the reaction at the desired time. The reaction is between helicase and DNA. I have provided all necessary conditions for the reaction to...

30 March 2016 9,358 1 View

At what pH I can cross link the substituted Cys residues in a subunit of the enzyme FoF1 ATP Synthase?

I have a double mutant aG218C/aI248C of the enzyme FoF1 ATP Synthase. Now I am thinking of cross linking these two substituted Cys residues in the a-subunit of the enzyme. At what pH I should do...

13 July 2015 2,657 2 View

Why did E.coli cells became unusually dark in color after French Press?

I was trying to break open the E.coli cells which I have grown at 37 degrees Celsius in LB for 8 hr. The cells were stored at -80 degrees Celsius overnight. Next day after doing the French Press,...

04 June 2015 9,641 4 View

Wjy does the cloning of a gene mutation causing defect in DNA methylation in bone marrow cells cause no leukemia?

A particular leukemia is associated with a defect in DNA methylation system caused by a particular gene mutation.The cloning and transduction of this gene in normal bone marrow cells cause...

05 May 2015 1,734 4 View

How can we use Fluorescence study or Circular Dichroism to study the kinetics of protein folding?

If we consider the two state model of protein folding,then how can we experimentally test this model?

18 April 2015 8,350 9 View