Why my RNA is not good?

I am working in a laboratory with Cov19. Extraction is doing kit but it isn't good and the amount RNA, 260/280 is higher than 3. Can someone help us in this regard?

12 September 2020 2,827 7 View

Hello . Is there any stereo video dataset to produce depth ?

I'm going to produce depth video using a video with the right camera and a video with the left camera,(I mean two videos taken with the stereo camera).

16 October 2019 5,014 3 View

Proving the formation of NiCoFe2O4 nanoferrite by XRD?

Hi I synthesized NiCoFe2O4 nanoferrite. I used XRD to prove the formation of NiCoFe2O4 nanoparticles. I understood that the position of the peaks is correct. I mean that the nanoferrite powder...

07 December 2016 7,735 16 View

Hydrogen peroxide effect on vitamin c?

does vitamin C and H2O2 have any chemical reactions which will prevent us from detecting Vitamin C?

15 August 2016 1,715 3 View

I have trouble in detecting my protein of interest in total cancer cell line lysate in western blot. How should I solve this problem?

I can detect beta actin band and my cell line is MDA-MB-231 breast cancer cell line.

29 September 2015 7,619 20 View

How can I find a displacement table from stress-strain curve in order to use it in damage evolution in Abaqus?

I'm working on uniaxial tensile test for an epoxy and crack propagation in it. I have data from my lab work and now I would like to model it in Abaqus. I made the bone shape sample and everything...

03 February 2015 8,650 1 View

What is the difference between the prevalence of insulin resistance found in those with NASH versus those with Hepatitis C?

NAFLD shares many overlapping features with the insulin resistance syndrome, also known as "Syndrome X" including central or visceral obesity, type II diabetes, and dyslipidemia. Of course, NAFLD...

14 September 2014 882 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View