What is the most reliable method for determining protein concentrations extracted from adipose tissue?

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How to do a moderation analysis with 3 dummies (created from 4 groups) in SPSS?

Hello, I am writing a thesis where I want to test the moderation effect of 4 groups (that I transformed into 3 dummies), on the main impact of the independent variable on the dependent one. The 3...

10 June 2024 6,497 12 View

How to analyze organizational performance in international exports?

factors regarding organizational performance international exportations

30 April 2024 6,866 2 View

Comment analyser la performance organisationnelle dans les exportations internationales ?

RAS

30 April 2024 9,479 0 View

ITC data interpretation?

Hello I've collected ITC data and I'm not sure if this data looks correct. This was an experiment meant to understand the properties of an enzyme thought to be active as a dimer. The sample cell...

06 February 2024 6,452 4 View

Please can you recommend a PhD topic in Accounting?

I am interested in joining the doctoral Programme at my University and It requires a topic and concept paper. I have been reading around the area of blockchain technology and its impact in the...

07 March 2022 8,293 3 View

Could someone help me troubleshoot my NOS assay protocol for skeletal muscle extracts, please?

I am trying to measure differences in eNOS activity in skeletal muscle in sedentary and exercised mice, following the conversion of 3H-arg to 3H-cit, and assaying the samples with and with out...

31 January 2021 8,521 1 View

Primers not working with embryos gDNA but do with adult gDNA?

Hi all, it happened a very strange thing that never happened to me before. I run a PCR with primers for genotyping zebrafish embryos after CRISPR-Cas9 injection. I tried changing different...

04 October 2020 7,003 3 View

Family Holiday: what are the best destinations?

I am a master student of tourism and I am working on my Thesis. i am interested in family tourism and I would like to discuss about the characteristic of a family tourism destinations. To compare...

15 April 2020 4,304 7 View

Is there a metabolic connection between malonyl coa decarboxylase and δ-aminolevulinic acid dehydratase?

I am looking for different (out of the boxy) causes that would magnify adverse reactions in medication administration. Along with polymorphisms in the CYP enzymes, I believe FAOD's, as well as...

29 March 2020 4,349 0 View

How can I best preserve the photostability of mCherry in cryosections?

We have dissected tissue from P3 mice stably expressing a protein tagged with mCherry and fixed it for 4 hours in 4% PFA (pH 7.3) at 4°C. tissue is then frozen in OCT and then cut. and mounted on...

04 September 2018 2,969 1 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View