This question was extracted from the information from the journal “Tenants of Specimen Management in Diagnostic Microbiology” written by Rajeshwar Reddy Kasarla and Laxmi Pathak. The journal has relayed that most samples in Diagnostic Microbiology used for bacteriological examination or virus isolation were incubated, refrigerated, or incubated. Once the sample is received to be processed, what should be done to bring the sample back to its optimal temperature?
When transporting samples for bacteriological examination or virus isolation, it's crucial to maintain their integrity by preventing temperature fluctuations that could affect their viability and accuracy of analysis. Gradual warming at room temperature is a recommended method because it allows the sample to adjust slowly without experiencing sudden thermal shocks. This approach is especially important for delicate samples, as rapid changes in temperature can lead to cell lysis or denaturation of proteins, compromising the results of the examination. Using a water bath further enhances the control and gentleness of the warming process. Water baths are designed to provide a stable and uniform temperature environment, which is ideal for bringing samples back to their optimal temperature. By setting the water bath to the desired temperature and placing the sample container within it, you ensure that the sample warms evenly and without the risk of overheating. Continuous monitoring of the sample's temperature during the warming process is essential. It helps to prevent any unintended temperature fluctuations and ensures that the sample reaches the optimal temperature required for accurate bacteriological examination or virus isolation.
References:
Khandpur, R.S., (2019). Compendium of Biomedical Instrumentation, 2099–2101. https://doi.org/10.1002/9781119288190.ch398
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