What is the most efficient method for redissolving protein pellet. Which buffer can be used for resuspension for proteomic work. Esp 1D and 2D electp

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Bhairavi Vajaria

Dissolve your protein pellet in 8 M urea, 2% CHAPS, 50 mM dithiothreitol (DTT),

0.2% (w/v) Bio-Lyte®3/10 ampholytes if used for 2D electrophoresis.

Bhumika Upadhyay

thank you .. i am performing 2d and i could sucessfully resuspend pellet.. can someone please help me with IEF protocol for human saliva. i am using serum ief protoco, which doesnt seem to help as the proteins are all clustered..