Some researchers have criticized the efficacy of using multiplex ELISA in tissue homogenates (particularly for lack of sensitivity). My question is is this really an issue ? I am working with dorsal root ganglia
We have used mouse lung homogenates with Luminex technology or eBioscience flowcytomix that worked really nice. On the other hand, we had some problems with multiplex ELISArrys from SABiosciences with the same samples (some targets were not recognized, while for other the ELISAs worked quite nicely).
ah thank you for your quick response. to add to the opening post we are thinking of using quansys multplex elisa
I have also used the multiplex (Procartaplex) from eBioscience on serum and lung samples (20-plex), which worked out fairly well. A large number of the limit-of-detections for cytokines/chemokines that we were measuring (Th1 & Th2), were very low (1-5 pg/mL). Also last time I inquired, eBioscience no longer has the flowcytomix products.
One thing I would also like to suggest if you haven't already harvested the tissue (and you decide to use the multiplex assay), is to investigate the product setup beforehand. Some kits want you to use "special" buffers to resuspend your sample in. I personally didn't realize this (who does?) and had already stored my samples in RPMI, but everything worked alright anyway. Good luck.
Our lab uses patient serum and people have used Luminex assay (XMAP technology) without any issues.They have a whole panel of cytokines to choose from.You might have to check for the compatibility if your samples (which is tissue homogenate in your case) with the assay that you wish to use.
Meso Scale discovery offer a great system for multiple cytokine analysis, quick and easy.
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