When I extract DNA from my sample using CTAB method, after centrifugation, many oil molecules come out, which prevents the extraction of clean DNA.
Hi Mr. Shukrullo,
Fats are indeed rich contaminants and can be easily separated from the sample. It is important to isolate the DNA from the SVF (stromal vascular fraction) rather than the adipose tissue to avoid fat contamination. Before DNA isolation, performing collagenase type II treatment and two-step density gradient centrifugation can effectively remove fat cells and other contaminants, resulting in a cleaner DNA extract. Additionally, using a high-quality DNA extraction kit is crucial to ensure optimal DNA yield and purity, especially during the primary phase when DNA values may fluctuate.
Thanks and Best of luck!!
Dear Mohit Gupta
I will try the mentioned methods in the next experiments. Thank you very much for the reply.
Incubate the sample with ctAb at 37°C for 6-8 hours with minimal agitation. Alternatively, incubate with agitation on the shaker at 55°C for 30 minutes to remove fats and lipids. Then continue to use your regular isolation method.
Dear Ravi Prasad. Thanks for the reply. You mean the incubation period after homogenization should be longer, right?
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