Why I am getting multiple bands for protein of interest in western blot when my protein of interest is of 30.5 KDa?

I have utilized pET26b(+) NS2B-NS3 DENV2, and transfected it in E.coli. After transfection the selected cells were characterized based on the kanamycin resistance present in the vector. The...

18 February 2024 2,039 4 View