What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Why is my protein lysate not separated on a SDS gel?

Hello. I prepared protein lysates from Hek293t cells by using RIPA buffer. When I run my samples on a SDS gel, they don't separate. I have attached pictures of the gel after running it and of a...

01 August 2019 4,852 4 View

Is it okay to digitally magnify images of tissue stained via immunohistochemistry?

We took images at 10X of tissue from the central nucleus (CeA) of the amygdala, but those images included tissue outside of the CeA. In order to simplify things and keep from having to re-image...

10 May 2018 9,982 4 View

Fast image segmentation methods?

What is the difference in computational time between watershed segmentation and color threshold (in one color plane)? My images usually have one to three objects that needs to be separated from a...

30 May 2016 9,356 4 View

What is the most precise method for cell counting?

It seems that the method of cell counting with a TC20 cell counter by Bio-Rad is associated with with high variations. Therefore, I am searching for a better method to improve the accuracy. Can...

19 October 2014 3,735 7 View

Is crowd funding a viable alternative now that research grant awards are at an all-time low?

Crowd funding has been successfully used to launch many new inventions, musical and artistic projects. Will scientists now turn to crowd funding or is this merely a passing fad?

06 November 2012 2,809 13 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View