Is iron oxide (rust) a problem in DNA extractions?

Hello, I just started extracting DNA from some spiders, using 5-mm ball bearings to grind the specimens in a tissue mill. I had ordered the ball bearings on Amazon, and thought it was a good idea...

21 July 2020 2,271 6 View

Where to find resources to improve a self-designed psychometric instrument?

When our institution had to switch rapidly to remote instruction, professors who had not taught remotely before wondered what the best blend of synchronous and asynchronous activities would be....

27 May 2020 841 3 View

Why are surrogates only added at the time of extraction, not at the time of sample collection and processing?

I am working on a study on pharmaceutical fate and uptake in plants. We are wondering about analyte degradation during the initial transport, processing, and storage of the soil and plant samples,...

30 October 2019 3,540 1 View

Can anyone give me information on machine learning algorithm code?

Researching in the field of Abnormal Traffic Identification Methods.

26 March 2019 9,495 5 View

Can anyone suggest some qualitative research that has been done on low SES parental attitudes to literacy and lanaguge development?

I am studying this area for my masters in education thesis and would appreciate any suggestions on good articles/books/journals on this area.

06 October 2017 9,978 2 View

Any way to salvage data from merged colonies in a colony forming assay?

We attempted a series of colony forming assays of cancer cells for irradiation. However, due to some vibration issues from our incubator, the cells settled towards the center of the plate...

27 July 2017 5,593 1 View

How do heart-rate monitors work?

03 July 2017 1,651 8 View

What are the key morphological differences between male and female Tuta absoluta, a tomato leaf miner?

I am doing some biological studies on Tuta absoluta

04 April 2017 3,287 21 View

Issues with imaging Stain-free gels without using Biorad's imaging system?

I'm trying to do total protein quantification with Stain-free precast gels and after a lot of reading and many phone calls with Bio-rad, I am confident it's possible to read the gels and membranes...

15 March 2017 4,443 6 View

How can I distinguish each cell type in a tri-layer construct of fibroblasts (TIG120), HUVECs, and epithelial cells (MCF10A) by immunostaining?

I'm looking for specific markers for fibroblasts (TIG120), epithelial (MCF10 or MDCK) and HUVECs so as to be able to distinguish each cell type in a tri-layer construct I'm developing. Can someone...

24 August 2016 2,997 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to run raise regression in R?

if anybody know how to run raise regression in r, and raise parameter selection, please help me

24 February 2021 8,199 1 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View