Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Any suggestion on self-efficacy scale ?

Dear researchers, I am planning on conducting a research on the relationship between Language learning strategies, by Oxford (1990) and self-efficacy. I have found Morgan-Jinks Student Efficacy...

22 January 2021 8,432 3 View

I need to apply a questionnaire from an article. What statistical analysis do I need to use?

Hello, I found a survey about attitude toward language in an article which I find fit to use it in my thesis. The survey has 30 questions, which were put into 5 factors through factor analysis...

21 January 2021 9,419 3 View

Explain plasmid description?

Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....

14 January 2021 6,326 2 View

Why did I obtain abnormal staining by TUNEL assay?

I use Abcam TUNEL Assay Kit - HRP-DAB (ab206386) on mouse tissues. The staining showed a pattern that I expected to see with a specific layer of cells dying due to drug treatment. But the...

12 January 2021 144 1 View

Is there a generalized method to derive current-mode model of any DC-DC converters?

Hi all, As new DC-DC converters are being proposed, associated control method should be proposed and based on the small-model signal analysis of the DC-DC converters. Using average space state...

06 January 2021 7,247 3 View

Most appropriate fit of a standard curve in an enzym-linked lectin assay?

Dear all, I have a question regarding the most appropriate fit of a standard curve in an enzyme-linked lectin assay set-up to quantify the amount of mucin secretion in conjunctival explant...

03 January 2021 1,010 6 View

Can an airplane stay up in the the air without moving forward just like helicopter ?

How the lift and weight cancel each other perfectly for a helicopter to stay it motionless? Because its propeller do generate huge amount of lift due to air pressure plus a blend of air stream...

09 February 2021 3,990 14 View

I have a problem with sensible heat flux (H) iteration process in Sebal model?

In each iteration, I get the same value of H for different values of aerodynamic resistance and the temperature difference between two heights. Do the results make sense? Is it necessary to adjust...

07 February 2021 8,953 2 View

What reference do you recommend about doublet lattice method (DLM) coupling with finite element method (FEM) ?

I want to write a code related to the flutter of aeroelastic wing under unsteady load using doublet lattice method (DLM). I want a good reference with numerical examples or a book which learn me...

25 January 2021 7,488 3 View

How to extract the different components at a specific frequency between two spectra?

Dear everyone: Hello! Sorry that my question may take up your valuable time. Recently I have a technical problem in the data processing. We have conducted a series of wind tunnel tests in order...

18 December 2020 8,847 2 View

Any Reasearch article by by Kimura Akisato as detailed in the description below?

29 November 2020 2,728 1 View

What sensors are typically placed on aircraft, especially experimental?

In addition to sensors such as load cells, prandtl tubes, pitot tubes, which other sensors are put on experimental aircraft

19 November 2020 6,945 3 View

How to compare results of two different computational approaches?

I am looking for some analytical, probabilistic, statistical or any other way to compare the results of a different number of approaches implemented on the same test model. These approaches can be...

16 September 2020 5,902 7 View

Is there any difference in the instrument rating of an instrument mentioned in the technical manuals and operation manuals?

It has been seen that the instrument rating of an instrument mentioned in the operating manual is different from what is mentioned in the technical manual (Not all, but few). If we disregard the...

22 August 2020 853 3 View

What is the suggested courant number for an aerodynamic problem?

I am working with pressure based steady aerodynamics of a car. I found that, courant number plays an important role in convergence. In transient study it should not be greater than 1 but in...

28 July 2020 1,915 3 View

How to decide the proper number of elements in edge sizing for tetrahedral meshing ?

For hexahedral meshing, it is quite simple to divide one edge in a particular number of division. But for tetrahedral meshing it is not that simple. If we are using a complicated domain for...

29 June 2020 4,137 6 View