Thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR) is a fast and efficient method to amplify unknown sequences adjacent to known insertion sites in many organisms.
I'm looking forward to the best protocol for TAIL-PCR method,
What is your organism? The exact protocol will vary. Start with a basic PubMed search and that will get you started, there are LOTS of publications on this topic.
Basically, we are carrying out the experiment according to Lui et al. 1995, Lui and Whitter 1995, Lui et al 2007, and Session et al., 2002 (SALK institute) protocols performed on transformant rice, but the efficiency isn't enough.
Hi, you can try splinkerette PCR. We had very good results for a TT approach.
https://www.researchgate.net/publication/230686738_Transposition_of_the_miniature_inverted-repeat_transposable_element_mimp1_in_the_wheat_pathogen_Fusarium_culmorum
For for a general review of approaches you can check http://onlinelibrary.wiley.com/doi/10.1111/j.1742-4658.2011.08307.x/pdf
good luck!
Article Transposition of the miniature inverted-repeat transposable ...
Well, a low efficiency is expected since this is a "shot in the dark" sort of protocol. If you can pre-screen for transformed plants, then you will have a higher efficiency. Are they carrying a selectable marker like herbicide resistance?
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