Ammar Elakhdar

4 Questions 13 Answers 0 Followers

Questions related from Ammar Elakhdar

What is the appropriate polymerase to amplify gDNA greater than 10kb?

I am going to clone gDNA fragment > 10 kb into a pCR TOPO vector. I performed the PCR using KOD PLUS Neo polymerase for 3-steps PCR reaction, I am not able to find the specific size. Several an...

04 December 2020 2,140 5 View

How to discover/ identify cis- elements in the 3′ untranslated region?

We trying to find ways to discover novel gene or to identify the functions of novel gene that we have found. Gene (X) e.g., is gene decoded by eukaryotic translation termination factor 1 (eRF1)....

16 December 2017 1,736 1 View

What is the most accurate TAIL-PCR method allows for amplifying genomic sequences flanking transposition insertions?

Thermal asymmetric interlaced polymerase chain reaction (TAIL-PCR) is a fast and efficient method to amplify unknown sequences adjacent to known insertion sites in many organisms. I'm looking...

17 April 2017 3,420 4 View

The best method to isolate the dominant and homozygous mutant for the next generation sequencing (NGS).

Genetic mapping of mutations in model systems has facilitated the identification of genes contributing to fundamental biological processes. Using the whole-genome sequencing against pooled...

01 January 1970 500 1 View