10 October 2019 1 6K Report

I want to know the molecular weight of protease xiv. I searched in Sigma but not specified there. Anyone having experienced on enzyme can tell me. I also searched enzyme databases the information not available even EC number is not given and the database does not know.

More Wondimagegne Assefa Molla's questions See All
Which part of polypeptide is susceptible for reaction?

A given polypeptide protein has side chains and terminal (N- and C-). For any modification or conjugation reaction which part involved in reactions (side chains or terminals). Thank you !!

09 October 2019 1,001 2 View

How to avoid agglomeration either at the bottom or at the surface of polymer solution when mixing GO with polymer?

I want to prepare a polymer composite with GO as filler. When I tried, GO which was initially well-dispersed in the water start to agglomerate when mixed with a polymer. What should be the method,...

09 October 2019 5,148 1 View

For many days enzyme expriment, fresh medium of enzyme need to be replaced regularly. At what time interval the medium should be replaced ?

For many days enzyme experiment, fresh medium of enzyme need to be replaced regularly. At what time interval the medium should be replaced by new to keep activity of enzymes?

08 September 2019 2,185 5 View

Can anyone describe details of papain extraction from papaya?

I want to extract papain enzyme from papaya for my experiment. I do not know how to do that. I tried to google, my search was not enough. I need very basic knowledge from expertise. So, can you...

02 March 2019 1,125 5 View

I want to prepare Enzyme for experiments. Anyone can give me technical explanation?

For degradation of my biological protein sample I want to prepare Collagenase,alpha-chymotrypsin and Protease XIV. The solution is 0.5-75U/ml. The unit U/ml is my first time unit. So, can you...

01 February 2019 5,502 4 View

what are curve fitting parameters for perfect fitting?

Thank you

01 February 2019 7,923 2 View

Similar questions and discussions
MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close