the limit of detection is about 1 - 5 ng/band
When your gene of interest is very small, about 80-100bp, it is possible, that you have to do more cycles.
good luck ;-)
the limit of detection is about 1 - 5 ng/band
When your gene of interest is very small, about 80-100bp, it is possible, that you have to do more cycles.
good luck ;-)
the limit of detection is about 1 - 5 ng/band
When your gene of interest is very small, about 80-100bp, it is possible, that you have to do more cycles.
good luck ;-)
the limit of detection is about 1 - 5 ng/band
When your gene of interest is very small, about 80-100bp, it is possible, that you have to do more cycles.
good luck ;-)
the limit of detection is about 1 - 5 ng/band
When your gene of interest is very small, about 80-100bp, it is possible, that you have to do more cycles.
good luck ;-)
Hello,
Minimum concentration of DNA required to be visualized on Agarose gel depends on the dye used for staining. As you are using Ethidium Bromide for staining, 1-5 ng of DNA would be sufficient.
All the Best...
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