09 November 2016 3 8K Report

It is known that gordonia cell wall contain mycolic acid. i want to isolate dna from it.

  • Similar topics
  • Gels
More Edappayil Janeeshma's questions See All
Rules and regulations following for posting scientist B?

Dear friends, I really want to know the rules and regulations following for posting scientist B (reserved for ETB/SC) in Kerala. Kindly help me Based on notification No.MBGIPS/03/2022-A1 dated...

09 June 2023 7,532 0 View

How can i analyse GC_MS results using UPGMA?

I did secondary metabolites analysis (GC-MS analysis of methanolic extract) in Cd and Zn treated plants. As it did in different species of plants, I would like to know the similarity index using...

08 May 2020 1,489 0 View

How can I enhance the solubility of protein?

I isolated protein from leaf tissue using TCA and ice cold acetone. But some portion did not dissolve in buffer. How can improve the solubility?

13 April 2019 7,577 0 View

Please help me to choose an heavy metal tolerant maize variety which is available in india

I want to get a heavy metal tolerant maize variety for my research. I consulted many experts, but not get an answer. So please help me....

19 June 2017 453 6 View

How can I detect isoelectric point of a protein?

With acetic acid serial dilution i take an effort to detect the isoelectric point of an enzyme. I observed precipitation in PH 2.85 and 3.1.Of them which one want to taken as isoelectric point?plz...

17 January 2017 5,084 9 View

How can i calculate activity of immobilized lipase ?

i have to calculate activity of immobilized (using sodium alginate) lipase using pNPP assay

23 September 2016 9,743 11 View

The rules and regulations following for posting scientist B in Kerala

Dear friends, I really want to know the rules and regulations following for posting scientist B (reserved for ETB/SC) in Kerala. Kindly help me Based on notification No.MBGIPS/03/2022-A1 dated...

01 January 1970 9,561 3 View

Similar questions and discussions
Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close