I isolated protein from leaf tissue using TCA and ice cold acetone. But some portion did not dissolve in buffer. How can improve the solubility?
Dear friends, I really want to know the rules and regulations following for posting scientist B (reserved for ETB/SC) in Kerala. Kindly help me Based on notification No.MBGIPS/03/2022-A1 dated...
09 June 2023 7,531 0 View
I did secondary metabolites analysis (GC-MS analysis of methanolic extract) in Cd and Zn treated plants. As it did in different species of plants, I would like to know the similarity index using...
08 May 2020 1,489 0 View
I want to get a heavy metal tolerant maize variety for my research. I consulted many experts, but not get an answer. So please help me....
19 June 2017 452 6 View
With acetic acid serial dilution i take an effort to detect the isoelectric point of an enzyme. I observed precipitation in PH 2.85 and 3.1.Of them which one want to taken as isoelectric point?plz...
17 January 2017 5,083 9 View
i have to calculate activity of immobilized (using sodium alginate) lipase using pNPP assay
23 September 2016 9,742 11 View
01 January 1970 9,560 3 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,491 1 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,615 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,372 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,365 3 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,781 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 966 3 View
A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...
01 August 2024 7,051 5 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View