Histopathological examination never shows cut nucleus at different level
Hello!
You must consider that the size of the nucleus is arround 6 um. So, probably the thickness of your sections is bigger than 6 um.
Thickness may be 2 um but it does not matter how much thick section is . Nucleus may be cut down by sharp knife of microtome and should be visible as sphere cut at different levels.
When you cut down a tissue, the nucleus can be cut or not. It depends if the cut is in the same plane as the nucleus. The shape of the nucleus depends on how "the knife meets the nucleus".
Example: If the knife cuts longitudinally a colunar epithelium the nucleus are elongated, but if it cuts transversally the same epithelium the nucleus are spherical. The same for smooth muscle.
There is no mechanism as such through which the nucleus avoids sectioning during microtomy. It is a matter of size of the nucleus of your cell sample and thickness of the section you fix during microtomy that decides how frequently you get sections of nucleus on slide. By the way are these paraffin embedded tissue sections? Which cell types form your sample?
I have done histology of ovaries and ovarian follicles of fish, frog and mouse. I get not only nucleus also nucleolus in histological sections. Also try serial sectioning and number them consecutively, I am sure you will not miss sections of nucleus.
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