Can I use the CHARMM GUI input files for Gromacs in HPC?

Good day everyone! I am a Biochemistry undergraduate in the Philippines and to be very honest, we have no background in bioinformatics since we really focused on wet lab techniques + theory. Due...

15 February 2021 3,422 4 View

Cause of slow bromophenol blue migration and smearing while running SDS PAGE?

While running gels for western blotting, I am currently getting, what seems to be, the blue dye from my sample buffer migrating more slowly in sample lanes leaving blue streaks. These transfer to...

08 February 2021 5,317 3 View

Same sample, significantly different trace for DMF vs. THF for cyclic voltammetry?

Dear reader, I've been having trouble with my cyclic voltammetry recently, but I then had a break through and managed to get apparently "clean" results using dry, deoxygenated THF and/or DMF,...

06 January 2021 4,312 3 View

Can I use GROMACS for field-theoretic simulation of copolymers?

I need to know if I can use GROMACS instead of LAMMPS for my undergraduate thesis wherein I will generate phase diagrams of copolymers using FTS. Thank you so much!

22 November 2020 3,725 3 View

How to quantify lncRNA/miRNA in ribosomal depleted RNA-seq?

I did some ribosomally depleted RNA-seq in rat brain. Does anyone have recommendations on how to quantify/compare small RNA expression between samples? I have used RNA STAR to map reads back to...

11 November 2020 5,331 1 View

What are some essential steps when using THF for cyclic voltammetry?

I'm new to electrochem/cyclic voltammetry. I'm interested in measuring cyclic voltammograms of organic semiconductors in organic solvents, specifically, THF. I know that in principle I should be...

09 November 2020 2,298 4 View

At what stage of development do animals develop sentient?

I'm trying to find out in the development between being an embryo and an adult animal does the animal become phenomenally conscious? I'm especially looking for recommendations for articles on the...

30 August 2020 3,815 3 View

How to depolarize cultured primary neurons with KCl without them dying?

14 August 2020 1,488 8 View

Recommendations on model enveloped viruses that are easy and safe to propagate?

Hi everyone, We are synthesizing a virucidal material that will kill SARS-CoV-2 on contact. In these preliminary stages, we want to use enveloped surrogate viruses that are the easiest to...

08 August 2020 6,731 4 View

Where can I purchase fluorescent silica nanoparticles?

Hi all, I want to purchase fluorescent silica nanoparticles (with and without a functional group to make it hydrophobic) in powder form. A variety of size options would be key. Preferably, if you...

04 August 2020 7,440 6 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which reagent is best among 'Dimethylglyoxime" and "Dithiothreitol" for spectroscopic determination of Nickel ?

What I think is Dithiothreitol. As it is water-soluble and does not form a precipitate. But, the thing is it contains sulphur which is quite hazardous to aquatic animals.

01 March 2021 8,374 4 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

Is it mandatory to specify Subjects Combinations in Generalized Linear Mixed Models (SPSS)?

Hi all, I have data with two levels: lets think of the classic example of students and schools. When running the analysis in SPSS and selecting the Random Effects, we can select (we can or we...

24 February 2021 7,379 4 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

What type of SPSS analyses should I use for my Dissertation?

I'm currently working on my Dissertation and I'm trying to think of the best analyses that I should use for my study. I'm using three questionnaires for this and two different groups, 1st group...

22 February 2021 1,351 4 View