Max Myakishev-Rempel

9 Questions 7 Answers 0 Followers

Questions related from Max Myakishev-Rempel

Directionality of chromosomes?

By looking at a chromosome fragment, is there a way to tell, how it is oriented in the chromosome? I wonder if the genomic sequence in chromosomes is directional, for example maybe one strand has...

16 October 2019 8,236 2 View

Which brain areas in the rat to collect for RNA analysis of chronic alcoholism?

The rats will be subjected to chronic alcohol vs controls. The brains will be collected, RNA extracted and the levels of candidate genes will be analyzed by RT PCR. Which areas of the brain are...

10 May 2016 5,612 4 View

Could you suggest a gentle automatic physical way of cell culture synchronization?

I would like to produce synchronized adherent mammalian cell cultures for RNAseq analysis preferably using automated physical means, so the cells would be in most undisturbed and happy. I assume...

03 May 2016 9,640 2 View

Vertical hydrogen bonds in DNA?

I am looking for any references or explanations about vertical (longitudinal) hydrogen bonds in DNA, see the image attached. So far, I can not find any mentioning of them in the literature.

01 January 1970 9,412 2 View

What is the mechanism for the formation of long-unit tandems?

What is the mechanism for the formation of genomic tandems with long repeating units, like 40bp per unit? I don't think polymerase slippage can make such tandems.

01 January 1970 7,715 3 View

Experts - what is the rule for avoiding mispriming in PCR?

This is a quite advanced question - I am designing PCR primers to quantify a small copy number sequence in a mixture with similar sequences. How long can be 3' end match with undesired sequence...

01 January 1970 7,213 3 View

What is the shortest wavelength for sound in water?

I am writing a paper that mentions sound at the DNA scale. What do you think could be the shortest wavelength for sound in water, theoretically? Could the sound of 2 nm wavelength propagate...

01 January 1970 6,909 3 View

What are the typical conformation parameters of the B-DNA double helix in live cells?

I am studying DNA-related signaling and need to know the details of the DNA structure in live cells. To my surprise, I couldn't find any literature on that. Most of the studies were done on...

01 January 1970 4,294 0 View

Do purines in DNA have non-aromatic tautomers?

I would like to write a paper, where the central theme will be the biological significance of the figure shown below. The key statement there is that natural purines in the DNA of live cells are...

01 January 1970 5,487 2 View