I am read this sentence in artical paper >
(( Successive extraction with petroleum ether (6×50 mL), ethyl acetate (3×100 mL), and n-butanol (3×50 mL) afforded fractions))
I would interprete these numbers as: 6 times extraction with 50 ml petroleumether, 3 times with 50 ml ethylacetate and 3 times with 50 ml n-butanol.
I doubt whether 6 extrations is essential. I learned that after 3 extractions (nearly) all has been removed.
In addition to the reply above, note the order going from hexane (non-polar) to n-butanol (more polar).
I think it is better to make communication with the corresponding author for this article .
Thank you for all..
That is, the number of extraction times X (in) the volume of the solution used for extraction
Thank you doctor @Luay Abed
I read several research papers and found some of them say phytochemical examination of the plant after extraction, while others are before extraction after grinding the plant. Please Help...
08 September 2019 9,650 5 View
Distinguished researchers .. Please help with how to file and publish an international patent? Many thanks in advance
08 September 2019 7,623 4 View
Is it within the classification of Clarivate ? Journal of Reseach on the Lepidoptera
07 August 2019 8,196 4 View
To specialists .. Please help as much as possible. How can I measure TLC for a particular plant? Did you extract it with a particular solvent or what?
07 August 2019 8,515 5 View
Thanks for your answers
07 August 2019 3,065 5 View
An electronic application installed on the computer used to convert images to Word or Excel depending on the content of writing. ( ABBYY FineReader 10 Corporate...
01 January 1970 6,044 4 View
Do you treat your students as a friend or force? Your opinion interests me.
01 January 1970 2,389 15 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I am a Petroleum Production Engg MSc student at Robert Gordon University in Aberdeen and I am in the process of selecting a topic for my MSc Engineering Investigation proposal. I would very much...
01 March 2021 7,396 4 View
The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
28 February 2021 9,464 2 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?
24 February 2021 8,219 2 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....
22 February 2021 6,560 3 View
Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...
21 February 2021 7,447 2 View