Why ecology system allows virus exsit?

27 February 2020 5,293 3 View

Why do they immunize mice by intraperitoneal injection?

Under what conditions will we use the method of intraperitoneal injection of vaccination in mice? In streptococcus suis research, some studies vaccinated mice by intrapetitoneal injection? why?

24 February 2018 8,500 6 View

A protocol for NKT cell depletion?

I am now planning to deplete the NKT cells in c57 mice and then injected with tumor cells. I searched online, and found some people used NK1.1, some used CD1d. I am now confusing as NK1.1 antibody...

20 December 2017 8,803 1 View

Does promoter-cre expression affect the the original gene expression?

I am confused by the Cre-flox system. For the conditional knockout (Cre-flox) system, Cre is expressed under a specific promoter. It is knocked-in using the homologue recombination, so the...

25 April 2017 9,244 3 View

What method can estimate bicycle traffic volume?

There are so many methods in estimating car traffic volume.However, methods about estimating bicycle traffic flow are very few.So I want to know some methods can estimate bicycle traffic volume.

16 March 2017 2,546 8 View

How to prepare 5ng/ml PMA from Sigma P8139?

Hi,I'm looking to prepare a working concentration of 5ng/ml of PMA from Sigma's PMA (P8139).How do I go about this? Please advise. I have also provided a link to the relevant data sheet...

21 February 2017 584 2 View

Which primer should I use if I want to detect the mRNA level of Akt in HCC cell lines?

Dear All, In my western blotting experiment I found a decrease level of both Akt and pAkt expression. So I want to check the mRNA level of them. I am wondering which primer should I buy for the...

16 October 2016 3,622 4 View

What is the minimum amount of starting material to use in flow-cytometry?

16 October 2016 2,440 3 View

What expression proteomics techniques to use in characterizing the protein profile of iPSC-derived leukemic cells?

06 October 2016 167 1 View

Nafion can't be used in high temperature in ORR testing, how can I solve it?

I want to coat some Pt catalyst on the glassy carbon electrode, and test it at high temperature (>140 degree). Usually, nafion is used to fix the catalyst on the GC. But as I know, nafion can't...

16 July 2016 2,190 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View