some articles mentionned that od600 of bacterial growth reached 50 to 90. is it of whole broth or of the measured volume (2ml) by spectrophotometer??
Not my field but do you know of any vis spectrophotometer that could directly measure such a large OD?
Paul Milham
dear Mr Paul
As i know max absorbance of spectrophotometer that i use it has max value of 3 because there is relationship between absorbance and transmittance, and exceeding the maximum it gives faults values
that why I'm asking i have no idea if there more developed equipments nowadays.
It is possible to get culture densities in excess of 50 (OD600) but this generally requires a fermentor or bioreactor with really excellent aeration, mixing, pH control, glucose feed etc. In a typical flask with E. coli you might get to OD 2-3 and perhaps up to 10 with special media and baffled flasks. But note that the actual OD measurements are not very accurate above 1 (and certainly not above 1.5) so these OD values are determined after dilution of the culture and assume theoretical linearity.
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