Is it wrong to report an unavoidable unblinded reading of tuberculin sensitivity test (TST), even if took appropriate steps to mitigate it?

We conducted a study to ascertain Mantoux reaction among diabetic patients. Since we conducted the work in a diabetic clinic, we found it difficult to blind the subjects (from investigators or...

04 May 2016 889 2 View

Can I perform a validation of scale into a different language but avoid conducting construct validity temporarily?

I conducted the validation of a scale into another language and it seems to mirror the findings of the original and other international results. However, I was not able to conduct a construct...

31 December 2015 8,840 1 View

Using the Theory Planned Behaviour framework, how do we identify which items to remove from the indirect measures of a construct in a questionnaire?

We compared the composite scores of the indirect and direct measures (attitude/subjective norm/perceived control). They were all strongly correlated. But in some journal articles, authors seem to...

05 June 2015 2,449 8 View

What do you conclude when you have a paired t-test result that is significant but with a high standard deviation (about half the value of the mean)?

The skewness and kurtosis of the data was within limits that can be considered normal (-1 to 1). Do you still conclude that the results are significant but stating that a larger sample size would...

04 May 2015 5,736 4 View

The Questionnaire Conundrum - Is there a way to ensure that a questionnaire is answered satisfactorily well?

Recently, we embarked on a research project that involves the assessment of the intention of primary care physicians in providing sickness certification to patients. We rigorously conducted pilot...

01 February 2015 3,502 10 View

Which would be the best journal to publish health service related research work?

Currently working on sickness certification behaviour amongst physicians using a psychological scale. Was wondering which would be the best journal to submit my research findings to. Any suggestions?

10 November 2014 233 2 View

Do I need any permission to use a validated questionnaire that is widely avaliable in the public domain?

I was wondering if I need to get permission from the principal investigator to use a questionnaire that is widely available in the public domain and published by the same researcher. If the...

10 November 2014 5,863 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

What is recommended and opened real-time or nearly real time geospatial data used in flood risk assessment and regional planning on a detailed scale?

I asked recommended real-time or nearly real-time data source for any parameters for flood risk on a microscale analysis for urban area cases in developing countries that had agglomeration urban...

18 February 2021 2,617 2 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Has anyone tried eBioscience Foxp3 / Transcription Factor Staining Buffer Set with TdTomato+ cells?

Hi all, I stained tdTomato+ cells (PBMCs and Jurkats) with eBioscience Foxp3 / Transcription Factor Staining Buffer Set for the foxp3 and I completely lost the signal of tdTomato. I tried to fix...

10 February 2021 5,863 2 View

How could a transcriptional factor purified as inclusion bodies (by using urea) exhibit a ratio 260/280>1?

I am working with a very unstable transcriptional factor. I use DNAse in the lysis buffer and I purify it as inclusion bodies, by using urea and 1M NaCl. After purification, I buffer exchange the...

05 February 2021 2,530 3 View

Endothelial Functional Assays & Conditions?

Hi everyone, I would greatly appreciate it if someone can shed light on certain endothelial functional assays and their conditions. I often notice that protocols are run with serum-free media, or...

05 February 2021 7,276 1 View

No signal/bands observed on western blot for ERK signaling?

My question is regarding a western blot experiment that I am performing to observe pERK signaling by stimulation with VEGF ligand (VEGF165 and VEGF121) on MDAMB231 cells and SW620 cells (both...

30 January 2021 1,039 3 View