Can anybody differentiate these? They look very similar but with a different way of presentation.
Dear Atul,
All are similar in the context of their measurement. But, IC50 is used generally for chemical assays like oxidation /reduction or other colorimetric/ fluorimetric assays where one chemical concentration inhibits 50% of these processes (Even for CFU measurements for Bacterial growth inhibition).
Whereas LC/LD50 will be applicable to animals (rats, mice..etc). In between LC50 and LD50, certainly LD50 will be used to represent the drugs /chemical which will kill 50% population of animals in single oral administration & where as LC50 will be used to show the concentration of a chemical in air exposed for 4hr to kill 50%of population.
So, According to OECD LC50 shows the lethal conc. of a chemical in air exposed for 4hr to kill 50% of population during observation (generally 14 days) where as for single oral/dermal dose of that chemcial to kill 50% population generally referred as a lethal Dose LD50.
LD50 also referred as Quantal response.
I hope it will clear your doubt.
Dear Atul,
All are similar in the context of their measurement. But, IC50 is used generally for chemical assays like oxidation /reduction or other colorimetric/ fluorimetric assays where one chemical concentration inhibits 50% of these processes (Even for CFU measurements for Bacterial growth inhibition).
Whereas LC/LD50 will be applicable to animals (rats, mice..etc). In between LC50 and LD50, certainly LD50 will be used to represent the drugs /chemical which will kill 50% population of animals in single oral administration & where as LC50 will be used to show the concentration of a chemical in air exposed for 4hr to kill 50%of population.
So, According to OECD LC50 shows the lethal conc. of a chemical in air exposed for 4hr to kill 50% of population during observation (generally 14 days) where as for single oral/dermal dose of that chemcial to kill 50% population generally referred as a lethal Dose LD50.
LD50 also referred as Quantal response.
I hope it will clear your doubt.
I would be cautious about using IC50 values for bacterial growth inhibtion data, it depends on the shape of your dose-response curve. In my experience performing liquid microtitre plate based growth inhibition assays with yeast, the curves are not the typical sigmoidal curves you see in other dose-response type experiments. Most software will model the dose-response data to a sigmoidal curve, however if the ascending portion is very steep, the error associated with the caluculated IC50 value often exceeds the IC50 value itself. In this case I tend to favour MIC values. You should always plot your data and let the shape of the curve determine what value you will calculate/report.
what is difference between IC50 and LC50 for yeast growth?How could i do it for assaying cytotoxic?
LC 50 mean mean lethal concentration. It means the concentration of the toxicant that produced 50% mortality in the test animals used in the bioassay test. LD 50 means mean lethal time or than period of time in hours or days that produced mortality of 50% in the test animals
The LD50 is defined as the lethal dose at which 50% of the population if killed in a given period of time; an LC50 is the lethal concentration required to kill 50% of the population.
The half maximal inhibitory concentration (IC50) is a measure of the effectiveness of a substance in inhibiting a specific biological or biochemical function.
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