How can writing tasks be designed or redesigned to accommodate the diversity in a multi -level ESL classroom?

Designing writing tasks in level- playing conditions of diverse groups in ESL settings calls for structural adjustment with the established notions of task design features. Yet , the culture of...

21 October 2020 9,469 6 View

How to Convert large fastq file to fasta?

Hello, I'm new to bioinformatics, my NGS dataset consists of 50 FASTQ files and each file is of size 10-15 GB.. I need to convert these FASTQ files to FASTA files. P.S. I am using google colab I...

02 July 2020 6,224 2 View

RNA Quantity & Quality extracted from Escherichia coli in M9 supplemented with different sugars?

I am trying to extract RNA from Escherichia coli grown in M9 minimal media supplemented with different sugars, however, every time the RNA extract ranges from 25 to 50 ng/ul. I am using the Qiagen...

23 October 2018 992 2 View

Is there an online source to know the equivalence of each OD to cfu/ml for Escherichia coli?

Is it the same for all media or differs from LB to minimal media? Doesn't OD correlate to cell mass? To be sure should i do a viable count for the growth of E.coli in specific media?

20 October 2018 2,588 3 View

Best Gel documentation system ?

02 April 2018 6,383 4 View

Electroporation of cripr cas9 by gene pulser?

Kindly I need to do transfection for canecr cell lines by Crispr cas 9 from santa cruiz (downregulation and overexpression) , I wonder if cuvette 0.2 will work with it as I don't have 0.4 and the...

28 March 2018 3,398 2 View

Size of electroporator cuvette size for mammalian transfection ?

I am trying to transfect SNU449 cell line by GFP plasmids using genepulserX Bio rad electroporator. I wonder if I can use .4 cm cuvette and voltage needed ! and what is the cell size of SNU449 !

21 March 2018 7,864 3 View

No title

I am trying to do transfection of SNU 449 hepatocellular carcinoma cells by GFP plasmids using Bio Rad micropulser electroporator ! according to its protocol it nables safe and reproducible...

13 March 2018 8,271 3 View

How to combine Response spectrum analysis results with static structural results in ansys workbench ?

I am working on load combination effects on structure and i need to combine the results of response spectrum analysis with static structural ... How can i achieve the desired results ?

10 October 2016 3,322 4 View

Please, I would like to ask about the calculation of the annealing temperature of that primer?

07 August 2016 9,183 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View