Hi everyone,
After RNA extractions and DNase treatments, I measured my samples for RNA yields in NanoDrop. On average they are 10ng/ul (ranging from 2ng/ul to 29ng/ul). Very low. 260/280 ratio between 1,7 and 2,1 for some samples...
Would this numbers be OK to run on a denaturing gel to see the 28S:18S rRNA bands?
Would it be better to stain the gel after running it?
Any protocol or suggestions/comments on this will be much appreciated.
Nice week !!
Ignacia
Md. Mahiuddin Zahangir
Hi
Your RNA yield is too low to run on gel. You can try 50ng or 100 ng in total t run on gel. I usually dont use the RNA having 15ng/ul concentration for RNA work.
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Gels
More MaríaIgnacia Meza Cerda's questions See All
Similar questions and discussions