Giemsa stain is one of the best known histological stains which make the nuclei dark blue and the cytoplasm blue to pink; according to the acidity of the cytoplasmic Giemsa stain is used in Giemsa banding, which is commonly called as G-banding, to stain chromosomes and often used to create a karyogram (chromosome map). It can identify chromosomal aberrations such as translocations and rearrangements (Methods in Enzymology, 2010).

Following article may be useful:

http://www.science.smith.edu/cmbs/wp-content/uploads/sites/36/2015/09/Chromosome-prep-and-stain-2014.pdf

https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/giemsa-stain

Giemsa staining

it is a very easy method

1. Fix air-dried samples in methanol for 10 min.

2. Air-dry until all methanol has evaporated.

3. Stain in coplin jar containing 5% Giemsa stain (diluted in tap water) for 20 min.

4. Wash sample in large beaker filled with tap water until excess Giemsa stain is removed.

5. Air-dry and examine under microscop

Luis

Thank you for all of you i'll try those techniques.

Cordially