Iam trying to check floiurescence in bacteria, but iam failed?

I am working on E.coli bacteria after growing bacteria in LB I centrifuge it at 7000 then resuspend it and add 2 ml deionized sterile water and 19 microliters gold. In the confocal machine, there...

14 November 2019 2,774 3 View

Cell lines to evaluate cytotoxicity of drug?

Hi Everyone, I am working on some drugs for HSV. I am wondering what are the best heart, liver and kidney cells lines to evaluate cytotoxicity of my drug on heart, liver and kidney...

17 June 2019 7,591 2 View

Hi , I wanted to collect exosomes from cell culture media through ultracentrifugation.but i couldn't see pallet. Why?

I collected media in two bottles and centrifuge for 800g RCF for 10 minutes . i couldn't see any pallet , then i went for 2000g for 20 minutes still nothing what should be the possible reason?

27 December 2018 5,642 3 View

I want to collect blood of normal mouse after treating it with Ag to check drug uptake, What is best way to collect blood?

doing animal model, for cancer related research

19 December 2018 2,428 3 View

My pure anabaen sample is contaminated with chlorella vulgaris. How to get rid of chlorella and keep anabaen pure?

I am facing problem regarding purification of anabaena. i have used serial dilution and plate streaking method. But everytime the culture is contaminated with chlorella vulgaris. I have to perform...

12 November 2018 7,869 3 View

I fabricated GNP by reduction of sodium citrate but its colour is not bright red ,, what might be the reason ?

Fabrication of GNP

28 May 2018 9,702 1 View

How wind speed is mathematically related wind power???

How wind speed is mathematically related wind power???

23 February 2018 1,959 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View