These exosomes from cancer cells can cause issues relate to immune response, detrimental effect (due to functional exosomes).
The plasmid that was transformed originally is correct (sequenced). I am expressing different gene targets from the yeast genome to optimize my protein. When I sequence the incorrect size, it...
01 July 2024 2,356 6 View
I want to check my protein expression but the sample (target protein) on my SDS PAGE shows a little difference as compared to the control. I measured the band density through IMAGE J but the band...
01 July 2024 1,841 3 View
I made a primer that gives 2 bands in every 15 samples in ZS11xSiloga BC3F1 genration. I thought that it might be technical problem, i again ccheck it with BC2F1 generation so i got homozygous...
21 March 2024 9,382 0 View
Actually, I am trying for months to make a primer of a Clubroot resistance gene (CRa). CRa gene is found in three Brassica species. My aim is to make a primer that can amplify this one gene in all...
06 March 2024 9,877 4 View
extraction techniques best food fortification from ginkgo biloba leaves health benefits seed for cultivation of ginkgo biloba plan
22 January 2024 2,303 0 View
Some studies have shown coffee and other caffeinated beverages can reduce iron absorption. For example. Drinking a cup of coffee while eating a hamburger can reduce iron absorption by up to 39%....
26 December 2023 3,278 2 View
Autologous donations are blood donations that individuals give for their own use – for example, before a surgery.
26 December 2023 4,494 2 View
IgG is the only class of immunoglobulin capable of crossing the placenta.
29 October 2023 7,735 2 View
I hope this message finds you well. I am currently working on a research project focused on MD simulation study of drug desorption behavior on a boron nitride nanosheet with a non-covalent polymer...
28 September 2023 4,514 0 View
I am writing to seek assistance on a matter related to n2t file generation. I am using GROMACS and charmm36 force field in my work. I am covalently attaching a short polymer(poly-peptide) to a...
30 August 2023 8,023 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
what are the top 3 challenges to the advancement of the field of Radiogenomics in cancer research? is it the availability of easily available low-cost matched imaging and biosamples with clinical...
03 August 2024 5,828 4 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View