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More than 33 million infections with more than one million deaths worldwide as of 29 Sept 2020, the Wuhan pandemic is a around 10 months long nightmare and showed no signs of improvements. The...

28 September 2020 9,840 6 View

Role of Ivermectin in managing COVID-19 pandemic...?

03 April 2020 4,784 11 View

Use of Large Waveguide Port Extension Coefficients?

I have tried to design a third iterated Sierpinski Gasket fractal antenna of a standard 88.9 mm height for multiband operation. This antenna resonates at 0.52 GHz, 1.752 GHz, 3.712 GHz and...

28 December 2019 6,168 2 View

Like Trees of Delhi which gives easy keys for tree identification in Delhi is there any book of similar kind for Uttarakhand State flora ?

15 April 2017 5,515 8 View

How to solve Partial differential equations by using finite element method?

I am trying to solve set of PDE's by using Gelarkin Finite element method for gaseous phase adsorption of VOCs on activated carbon for drawing breakthrough curves. I have used a quadratic...

03 January 2017 5,777 4 View

Has anyone run dsRNA immunoblots?

Hi everyone I am trying to run an immunoblot for dsRNA molecules of specific lengths. I have been running 5% PAGE gels and transferring the RNA using semi-dry transfer @ less than 10V. Note: the...

22 August 2016 3,101 1 View

Ways around expressing a protein which is seemingly toxic for the cells at higher concentrations in mammalian cancer cell line?

Hi everyone Background: Expressing a membrane protein in A549 cells (lung epithelial cancer cell line) using a expression vector under the constitutive CMV promoter. I am able to detect the...

29 March 2016 1,027 3 View

How to express a fusion protein where one of the protein is a receptor protein?

28 January 2016 6,864 4 View

Disadvantages of using long questionnaire?

20 January 2016 771 8 View

Any suggestion for running generalized estimating equation (GEE) in SPSS?

26 November 2015 5,414 7 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View