How do you find the melting point of a material using the two-phase method with DFT+MD?

Dear all, How do you find the melting point of a material using the two-phase method with DFT+MD? Thanks, Uthpala

02 September 2019 9,650 6 View

What are the best performing real-time machine learning models for anomaly detection?

Specifically, I want to know real-time machine learning models that are capable of identifying anomalies considering streams of data with multiple features (i.e. multivariate time series). I have...

04 June 2019 9,780 3 View

Why does a peak as water apper in GC-MS spectrum at retention time 21.9 & 22.1 when organic solvent analysis with following condition?

Injector @ 300 celcius Oven 100 (3min) Rate 10 C/min Final 300 (2min)

06 February 2019 3,858 3 View

When calculate the volume of the Alo vera gel should I consider the water amount or Is it ok to add just the volume of Aloe vera gel with 98% water?

I want to make thin films with Banana starch by adding different concentrations of Aloe vera gel 2%,4%,6%. So Aloe vera is having about 98% of water.When I calculate the volume of the Alo vera...

05 February 2019 2,591 2 View

Protein expression of Gal-genes in yeast?

Hi, I was carrying out Galactose induction experiments to determine protein stability. However, one of my wild-type strains expressed only a trace amount of proteins, which was unexpected as...

22 October 2018 6,859 4 View

How does distance between two electrodes affect reaction rate of electrolysis?

Hi, I'm doing a school project and was wondering what the effect of changing the distance of the two electrodes is during electrolysis? Thanks!

27 May 2018 7,128 3 View

How can I measure the intensity (power density) of a laser?

I need to measure the power density of a laser. What is the most appropriate equipment to measure the intensity of a laser beam?

10 April 2018 2,227 3 View

How to write the concentration of the media by mg/L or mg/l?

I found both forms in research articles. what is the correct way of writing?

02 October 2017 8,411 3 View

How is it possible a point mutation to become homozygous from Forwarding primer while being Heterozygous from the Reverse primer?

03 April 2017 3,135 3 View

What are good research topics in educational data mining?

I'm working on Educational Data Mining (EDM) area. I need good topics to do a research on EDM. Can u suggest some advanced topics?

01 January 1970 5,562 12 View

Covid 19 Pandemic ' impact on digital transformation?

Covid 19 Pandemic and digital transformation

22 February 2021 3,351 5 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Is the logarithmic transformation a necessary step in time series analysis ?

Hello, I'm checking if the log-tranformation of time series data is a necessary step; We all know the aim of such transformation, but in every study (in time series analysis) we found the authors...

15 February 2021 5,655 14 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

Mixed ANOVA with some normal and some non-normal data?

I am looking to run a 2x5 Mixed ANOVA to examine Sensory Processing Sensitivity in Highly Sensitive People (HSP) and Non_HSP. My Between-Subjects factor is HSP and Non-HSP. All participants tested...

11 February 2021 1,445 6 View

What could be the cause to have an E.coli DH10B transformed with pUC18 to grow in MKL + ampicillin but is not LAC+?

Hello everyone, I did a transformation of E. coli DH10B using pUC18 plasmid. The bacterias did grow on MKL medium with ampicillin but the colonies showed a white/pink colour and the medium was...

08 February 2021 9,720 3 View

After transformation of Dh5alpha competent cells I found a cloudy white ppt before plating. Can anyone explain this?

After transformation of Dh5alpha cells by heat shock I grew them at 37 in 200microlitre Terrific Broth (because I had it at hand) and then before plating I found cloudy white ppt in the cell...

03 February 2021 7,867 3 View