I'm working on a research and the surface staining procedure advises that we should use the staining buffer with FBS for washing after incubation with fluorescent-conjugated antibody. I want to know the scientific reason for this
To removing not covalent connected anti bodies.
Regards.
I would guess that the high concentration of albumin in the FBS works as a blocking agent to reduce non-specific binding of antibodies.
following
FBS works as a blocker which eliminates non-specific binding of the antibody. In other words, FBS reduces a background and gives clear results.
For blocking and to avoid non-specific binding/staining.
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