I used 0.5% Aq. AcOH solution to dissolve chitosan and derivative to evaluate their antimicrobial activity and performed anti-bacterial activity and antifungal activity using the broth dilution technique in 96-well micro-trays (NCCLS, 1993) and the antifungal screening method (NCCLS M27-A2 protocol, respectively. The cytotoxicity tests of prepared chitosan derivatives were assessed by MTT assay.
Now reviewer have following comment.
kindly help me in replying the comments of reviewers
comment of reviewer: if the derivates are still not soluble in water how are they be used in the kind of treatment proposed? What it's the implication of using acidic media in antimicrobial or cytotoxic treatment ?
It's just as the reviewer commented. Acetic acid will have an impact on biological assays - both by itself and in combination. It will be impossible to isolate the effect of just chitosan and its derivatives if acetic acid is need for solution.
More importantly and tho' you didn't identify the "treatment" the reviewer mentioned - there is very unlikely to be any application for chitosan in 0.5% acetic acid therapeutic or otherwise. You should be addressing efficacy of chitosan in isolation - perhaps as a surface coating.
I don't think you have a reasonable response.
Thank you for your response @Phil Geis. I checked chitosan and its derivative's antimicrobial and cytotoxicity on MCF-7 and L-132 by dissolving them in 0.5% acetic acid to make solution for biological evaluation. but reviewer asking me the implication of 0.5% acetic acid in such cancer treatment as my derivatives are showing higher cytotoxicity on L-132 and MCF-7. further we checked their effect on normal cell lines, but no derivatives and chitosan showed any significant effect on MCF-7 and L-132 cell line. Chitosan and derivatives had IC50 value of around 3000 ug/mL on normal cell line which indicates that chitosan and derivatives are non toxic to normal cell line but selectively toxic to MCF-7 and L-132.
Now could you please help me to give appropriate answer to reviewers comment.
Thanks and regards
The reviewer is apparently concerned that the solution is not useful for clinical application due to the acetic acid - that one can not for example inject acetic acid internally. You need to defend the safe use of acetic acid internally AND that chitosan remains solubilized when delivered to the tumor in the body. That is - introduction of the solution into the body will result in a body fluid matrix that doesn't consist of just dilute acid acid. If chitosan falls out of solution in that matrix - how will it impact tumor.
- Badges
- Science topic
- Similar topics
- Geoscience
- Atmospheric Sciences
- Atmosphere
- Air