How to perform antimicrobial activity of Chitosan Schiff base as it is insoluble in any solvent. Please could anybody guide me?
: In some of papers I have found that they used powder while performing antimicrobial activities.
Pruthviraj Limbachiya The solubility properties of chitosan depend on the degree of deacetylation, where the higher the degree of deacetylation, the higher the solubility, and the low degree of deacetylation shows poor solubility, and the solubility of chitosan depends on the amount of protonated amino groups in the polymeric chain, so organic acids can Such as acetic, formic and lactic acids can dissolve chitosan, and the most used solvent for chitosan is 1% acetic acid at pH 4
Sir my question is in what solvent should I perform antimicrobial activities as in most of the paper they haven't mentioned the solvent in which they prepared solution. In some of the paper I have seen taking solid Chitosan and it's Schiff base to perform antimicrobial activity.
So I'm bit confused whether I should perform antimicrobial activities taking solid Chitosan and derivatives suspending in DMSO or should I go with Chitosan and derivative's solution dissolved in 0.5% acetic acid aq. Solution.
-Thank you for your response.
Pruthviraj Limbachiya I put acetic acid with chitosan to dissolve the chitosan, and the concentration of the acid is very weak, so it will have no activity against the acid used, only the activity of chitosan
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