1. May I do a paired-end 150 bp (2x150bp) sequencing for the ATAC-seq library? Or do you recommend other read lengths?

2. Please correct me if I'm wrong. If I am using 2x150bp, these 2x150 bp fragment sequences will be further trimmed down to remove adapters before genome alignment, right? I have seen articles where they did 2x50bp, 2x100bp, and 2x150bp. That is why I'm confused if I should do 2x150bp, 2x100bp, or 2x50bp.

Looking forward to your responses and thank you in advance.

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