08 March 2017 2 3K Report

Chromatin purification from paraffin-embedded sample for genome-wide epigenetic studies (pathology-tissue chromatin immunoprecipitation). What is the ideal protocol and the main factors that may interfere with the execution of the method?

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It was published in the Journal of the American College of Toxicology in 1990, but the author's information does not appear in the journal

29 November 2020 2,554 3 View

Has anyone been successful in seeding microglia from adult mice?

Hello everybody I've been trying to seed microglia from adult mice and I haven't been successful. I isolated them using Miltenyi Beads and that worked perfectly, passed them through the...

14 October 2020 4,572 3 View

Can I use the cryostat to slice brains that were perfused with 4% PAF and Gadoteridol?

Hi everyone Im trying to slice my mice brains with the cryostat, however, I haven't been successful, the tissue seems to stretch and then it breaks. Let me tell you the story about these brains:...

25 August 2020 6,479 4 View

How to transfect Tcells with siRNA?

Hi everyone, I am trying to transfect murine CD4+ T cells in vitro, using Lipofectamine RNAi/MAX however, even when trying troubleshoot, the positive FITC labelled control shows just about 3%...

03 June 2020 2,618 1 View

Alternate morphology of eletrocardiographic signals after heart mechanical injury..... ???

Hello, I need your help. We are studying electrocardiographic parameters in an experimental model of heart injury, and we obtained unexpected results. The ECG intervals alternated during the...

28 September 2017 2,581 4 View

What can I do to avoid contamination of my nested pcr?

Good day, I try to make a pcr nested but I have problems of contamination, I use as a template the negative product of the pcr in the second pcr and gives me positive. I thought that the pollution...

24 January 2017 6,992 2 View

Is there any reliable antibody against corticotropin-releasing hormone (CRH) for Western blot?

Hi guys, does anybody know any CRH antibody which would work for western blots giving a nice and specific signal, especially in brain tissues (PVN, hippocampus)? Any advice is welcome. Thank you.

27 January 2016 320 3 View

Sophie's choice: Random mate-pairs or gel selection in Illumina Nextera mate-pair preparation?

Hi guys! I'm sequencing and assembling a de novo invertebrate genome of about 0,8Gb. I want to make a lane run of Illumina nextera mate-pairs, but I'm having some difficulty on selecting the size...

06 January 2015 6,134 9 View

Can anybody recommend a good and specific p-CREB and CREB antibody for westerns in rat brain tissue samples?

Thanks in advance.

22 February 2014 7,262 4 View

The most appropriate nuclear and cytosolic fraction housekeeping gene?

Could anyone recommend the most appropriate housekeeper for nuclear and cytosolic fraction? I want to isolate both fractions from frozen rat brain tissues and am wondering what is the best and...

05 August 2013 8,453 6 View

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