Hello,
I'm currently working in a lab that routinely assesses the metabolic profile of obese/diabetic-like mice. When measuring HbA1C in the past, we used a manual boronate affinity column fractionation with mini-columns from Helena Laboratories which has since been discontinued. We have recently used an ELISA kit from Crystal Chem however it does not perform as well (we typically have low precision).
From my reading it seems that HPLC is the gold standard. In patients, ion-exchange HPLC with gradient elution is optimized for separation of HbA1C from normal, non-glycated HbA, however, with mouse hemoglobin having very different overall charges than human hemoglobin, it elutes differently off the column so that the glycated mouse hemoglobin does not separate from non-glycated hemoglobin.
I was told that the ideal method for assessing HbA1C in animal studies is boron affinity chromatography. From peoples experience, is this approach indeed the ideal method and if so, does anyone know of a core facility that can measure samples? I know that Trinity Biotech have such a system but it is not feasible for us to purhase the entire system.
Any advice is deeply appreciated,
Thanks!
Phillipe
I hope to contact with you dear, HPLC device was the best techniques, with more than one company and it's different from one to another due to the originality of makers.
Check Aleres Afinion POC-analyzer, it uses boronate affinity method,
I KNOW THE HbA1c IN HUMAN IN DIMENSION IS GOOD AND ALSO ARCHITECT MACHINE
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