Phillipe D. O'Brien

5 Questions 6 Answers 0 Followers

Questions related from Phillipe D. O'Brien

What is the ideal method for measuring mouse HbA1C?

Hello, I'm currently working in a lab that routinely assesses the metabolic profile of obese/diabetic-like mice. When measuring HbA1C in the past, we used a manual boronate affinity column...

04 April 2017 8,740 4 View

HFD-STZ NIDDM mouse model: Do insulin resistant HFD-fed C57BL/6J mice retain their insulin resistance post-STZ injection?

My question is related to the C57BL6 HFD-fed, streptozotocin-injected mouse model. Is there evidence indicating that these mice retain their insulin resistance post STZ-injection? In our model,...

09 September 2015 6,465 6 View

WB troubleshooting: How to block interfering IgGs that mask your target protein?

I am currently having issues with my immunoblotting which I feel can be resolved by using an alternative blocking buffer/agent. There appear to be numerous blocking agents available and I wanted...

12 December 2013 5,689 7 View

Protocol for indirect sonication using cup horn attachment

Our lab has recently acquired a sonicator (from Qsonica) with a cup horn attachment and I have been designated as the person to set it up. I have not been able to find much information from the...

07 July 2012 3,289 6 View

Seeking advice for preparation of lysates from mouse sciatic nerve & DRG for SDS-PAGE/WB.

I have recently started my first post-doc, switching from a pharmacology lab to a neurology lab, and am in the process of optimizing lysate generation from mouse nerve tissue as the current...

06 June 2012 7,011 4 View