I will use it in whole blood culture. What is the concentration so that it does not affect the cells?
They are apolar lipids
I am conducting adsorption experiments in a liquid medium, and one of my samples showed a decrease in Qe values with an increase in Ce. Has anyone encountered this behavior before? What does it...
26 June 2024 7,304 2 View
We are looking for a highly qualified researcher with expertise in advanced statistical analysis to contribute to a scientific article to be submitted to a prestigious journal by the end of the...
13 June 2024 569 4 View
It is very common to measure the pressure distribution inside the bearings with pressure probes connected to the oil film through holes in the bush. Once filled and pressurized, the probe lines...
12 June 2024 1,936 0 View
My name is Mary Elizabeth C. Bernardes -de-Oliveira or M.E.C. Bernardes-de-Oliveira. But, Maria there isn't! Please correct this for me.
27 May 2024 8,228 2 View
Hello Does anyone know how to program in Fortran or any other language that uses advanced calculation libraries? I need help making an executable to obtain results from 2 equations that use the...
20 May 2024 6,610 5 View
A recently published visual abstract should have the title "Word First Myelography..." instead of the error "Mielography". (the 2 other text references in the article are correct) I am changing it...
20 May 2024 8,134 1 View
In order to proceed with the quantification of an Azide Alkyne Cycloaddition reaction efficiency by using a fluorogenic probe with Cy5-DBCO, I first need to construct a calibration curve. The...
16 May 2024 6,266 5 View
Dear community, I am currently working on a proposal focusing on cancer-associated fibroblasts (CAFs) from malignant pleural mesothelioma (MPM). In my search among commercial cell line providers,...
05 May 2024 5,572 4 View
Hello fellow users, My research focuses on investigating whether financial stress leads to lower self-confidence mediated by connectedness, with financial stress measured in a parent/married...
01 May 2024 6,610 0 View
Hello fellow researchers, In my research, I investigate two members of the same household. The members of the same household share the same ID number (Nomem_encr). I want to retain household pairs...
28 April 2024 6,087 13 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...
25 July 2024 637 5 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View