Hello Rhamiya,

We used 150 uM of DPPH for 96 well plate. You also can try it. For further information follow our article links below:

Hope it will help you.

Best wishes....

http://www.ncbi.nlm.nih.gov/pubmed/26632175  http://www.ncbi.nlm.nih.gov/pubmed/25995827

HI Mahendran,

 I used 10µM final concentration. But make sure drugs or plant extract is clear and colourless.

Thank you. I will try that ..

Thanks.

It all depends on your experimental set up, especially the nature of the phytochemicals and its purity.

i agree Dr. Jai goash statement