I want to regulate the expression of some genes that are under the Tet regulation in transfected U20S cells, By adding a purified MCP protein. what is the ideal concentration to start with? and what is the maximal one?
Dear Sir/Madam, I hope that this email finds you well. My name on the paper entitled "Fertility Table and Thermal Requirements for the Development of Callosobruchus maculatus (F) Reared on...
27 April 2024 2,501 1 View
I am running EMSAs with different DNA probes (ss/ds/stem-loop etc.). I hybridized and purified the probes, and consistently see my hybridized and PAGE-purified stem-loop probes run faster than my...
06 February 2024 8,068 0 View
We have been trying to produce lentivirus with the Tet inducible system for a while, but we are getting no signal in the infected cells after infection. However, after transfection, there is a...
30 January 2024 9,755 3 View
Is it possible to calculate flood inundation level form sentinel 1 radar image?
10 December 2023 5,475 1 View
Using a 3-metre resolution RGB image, I can't distinguish between built-up and barren land/bear soil because they have nearly the same color texture. This makes it difficult for me to perform...
29 September 2023 5,533 2 View
The ChatGPT-3 (Generative Pretrained Transformer 3) is the third iteration of OpenAI’s popular language model. It was released in 2020 and is considered one of the most advanced large language...
23 August 2023 6,158 5 View
i work on vissim and before i run the simulation i want to know how to assign these periods
09 August 2023 5,690 0 View
I have a dataset that contains 500 field observations. The data dimension is 500 by 9 (that means 500 rows and 9 columns/variables). Among them, 8 variables are independent, and one is dependent...
09 August 2023 2,706 3 View
Hi, How to convert excel formula into matlab code?
17 July 2023 8,381 1 View
What is the Relation between Absolute Propagation Time (ns) with respect to the Received Power (dBm) at the UE end? I would appreciate if anyone can explain the two graphs and the parameters...
27 June 2023 1,155 5 View
How can the economic value of digestate management, utilization, and regulation, can spark interesting discussions and explorations? so one can list down the details and we can discuss in length...
17 July 2024 3,255 4 View
It is well known that the larger the organism, the less energy consumed per unit mass (Delong et al. 2010; Moses et al. 2016; Wells 2007). This is known as Kleiber’s Law, whereby the energy...
06 July 2024 8,736 0 View
I am validating the expression of several genes by qPCR to confirm transcriptome analysis. After many inconclusive runs, I realised my housekeeping gene (GAPDH) was downregulated in my...
27 June 2024 7,318 3 View
International maritime law, also known as admiralty law, is a body of laws, conventions, and treaties that govern private maritime business and other nautical matters, such as shipping or offenses...
08 June 2024 4,794 0 View
For my doctoral thesis I would like to carry out a gene expression analysis in the plant Melissa officinalis. For this purpose, I will examine various genotypes that are located in a field that is...
28 May 2024 5,833 1 View
Temperature regulation is of utmost significance in pediatric anesthesia due to several reasons:
11 April 2024 2,413 1 View
I tested gene expression by RT PCR followed by Western blotting to test protein expression. I get an inverse correlation with up-regulation at mRNA level and down-regulation at the protein level....
05 April 2024 4,679 6 View
"The BERQ can be used for free for research purposes. Please mail the authors to request reprints of the questionnaire." pls help me, i dont understand what is REPRINT while it FOR FREE for...
26 March 2024 6,904 0 View
Hi everyone, I performed an immunofluorescence (IF) staining for Ki67 using a validated, specific Ki67 antibody (Dako) and I see a clear upregulation of the protein expression compared to my...
14 March 2024 1,516 0 View
Hi, I have a general question about pathway analysis in scRNAseq. I'm under the impression that scientists pick pathways relevant to their study from the list of GO terms and present those in a...
13 February 2024 8,540 3 View