Proteins not stacking/ entering in a native PAGE. Can anyone help?

Hi. I am trying to study a biotin modification of my target protein in a crude cell extract and am interested to determine how much of my protein in getting biotinylated. After biotinylating my...

15 January 2021 2,214 4 View

What are the most important explanatory assets ?

06 December 2020 5,474 2 View

What are the methods of writing the interpretation ?

06 December 2020 3,267 3 View

Cell culture techniques: how to deactivate a viral infection while preserving cytokine storm markers?

Hello, I am trying to measure the cytokine storm markers following SARS-Cov-2 viral infection of lung cells and macrophages under different conditions. However, finding a safe way to deactivate...

05 November 2020 1,174 4 View

To deal with nutrient quality variability during feed formulation, what do you do from the following?

A: Use actual values from NIRS B: Use a margin of safety C: Use stochastic programming D: Do nothing about it

18 October 2020 7,123 5 View

How can I calculate the am ount required to prepare the following buffer?

ammonium sulfate 0.17 M - polyethylene glycol 4000 25.5% (w/v) - glycerol 15% solution How can I calculate the amount required to prepare the following buffer? I want to prepare 1000 microliter

03 October 2020 4,018 5 View

Why some viral proteins are unable to express and purify in pET28a expression and purification system?

Dear All, I am trying to clone and purify codon-optimized viral proteins using pET28a expression system. Even though I have the perfect insert in the recombinant plasmid, there was absolutely no...

24 September 2020 3,635 8 View

Non fluorescent E. coli GFP?

I Have a problem with E. coli GFP, I am culturing them in LB medium/ampicillin + IPTG, but not all the cells fluorescent after UV excitation (fluorescence microscope) although they grew in the...

15 September 2020 4,636 1 View

No title

can anyone know the range of the osteoclast activity range (resorption) in bone remodeling cycle in mice compared to humans. or any paper talking about this information. I need this specific...

14 September 2020 1,218 2 View

How are parents guiding their children with learning difficulties?

Parents' educational level is essential in raising the level of education

14 September 2020 6,306 4 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Can I download BibExcel for MacOs?

Hi everyone, I would like to ask how I can download BibExcel software for MacOs? There are some links which are supported by Windows but I could not find it for Mac. or is there any other software...

01 March 2021 652 1 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How to prevent ANFIS Model from negative prediction values in MATLAB?

I have input and output data set for "ANFIS modeling in MATLAB", and I am getting some negative predicted values of output in testing. However, the predicted values of output in training are...

28 February 2021 3,459 3 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

How does an AC-DC power supply Flyback Converter Based USB Charger Model using Matlab/Simulink looks like?

I am required to learn about Flyback converters and I got stuck not knowing to full design of the power supply flyback converter Based USB Charger Model using Simulink, especially the design model...

25 February 2021 5,435 2 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View