In place of skim milk powder what can I use for preparing Skim Milk agar?

I'm about to prepare skim milk agar media to isolate protease producing bacteria but unfortunately I don't have Skim milk powder. I need answers to the best alternative to use as a substitute....

16 October 2019 8,294 3 View

Data formatting issues in HIERFSTAT?

I'm trying an analysis on HIERFSTAT for the first time using a subset of my data. I have some formatting issue that I can not put my finger on because when attempting to run basic.stats and other...

05 December 2018 9,820 2 View

In order to assess the drug release profile of a nanoparticulate system, how can I know which is the suitable volume of release medium?

I am working with stimuli-sensitive polymer nanoparticles to deliver doxorubicin and I don not know the the volume of PBS that I should use to asses the release profile of the system.

30 May 2017 9,936 2 View

Can someone assist to interpret these two output results?

I have a model with one exogenous variable (X) which links to multiple mediators (4 variables M1,M2, M3 ans M4) and then both exogenous and multiple mediator variables links to endogenous variable...

14 June 2016 6,181 5 View

In need of a specialized function.

I need a function f(x) such that when f(x) is applied to any function's sub-functions, the result is the same as if f(x) is applied over the entire function. For example: f((a^2)b + ln(c)/d - 2^e)...

18 January 2013 6,150 7 View

Insuline and Alzheimer Disease

Ron Scott you may find information here: http://mp.medscape.com/cgi-bin1/DM/t/hDIhS0VoXWR0D2s0DUDl0Et&uac=126040DG "In an interview with Medscape Medical News, Dr. Dandona explained that the...

13 April 2011 2,850 10 View

auditive system and psychiatry

11 April 2011 3,335 18 View

Urban squares/plazas and tree canopy. Squares as urban forests.

I'm looking for examples of public squares/plazas with a high tree canopy coverage. Places that are classic 'plazas' in architectural meaning, function as places of large gatherings and events,...

01 January 1970 8,858 16 View

Is there a possibility to get involved in this project: Neuroeconomics?

I am from South Africa..Masters' student in Economics, also employed at the Department of Economics at University of South Africa. My studies focus on Neuroeconomics. It is a very interesting...

01 January 1970 4,273 4 View

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02 March 2021 3,060 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

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22 February 2021 9,932 11 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View