What is the growth medium for culturing Selenastrum capricornutum?

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Geometrically Derived Formula for Day Length Based on Latitude and Time of Year?

Is there any geometrically derived mathematical expression for the duration of the natural day at any latitude and any time of the year?

23 June 2024 9,895 2 View

Why there are a lot of colonies but no target fragment inserted?

I am going to insert a 6.3kb fragment into a 5.3kb plasmid. I use SAM1 digestion to cut the blunt end on the plasmid which is CCCGGG. I designed a set of primers to amplify the target fragment...

12 November 2022 7,352 3 View

How do I select substitution of codon without altering original function of the protein?

I want to mutate the ATGs of a gene, but I also want to not influence the original function of the protein. May I know whether there are criteria for selecting substitution of codon?

07 September 2022 1,719 1 View

Can mediation analysis be done only with indirect effect?

Hi, currently doing a mediation analysis for the following research framework (A -> mediated by C-> E) With such a framework, there's an indirect effect of mediation and no direct effect....

09 March 2022 3,673 3 View

How to successfully knockdown the SPP1 gene from MIA PACA 2 cells using siRNA?

I have been trying to transfect MIA PACA 2 using lipofectamine 2000. However, the real time PCR results (Ct values) are the same for the control and the experiment.

23 February 2022 687 3 View

How does water quality (pH and hardness of the water) effect the efficiency of foliar application of fertilizers?

pH can influence the efficiency for pesticides. We also know that hardness of the water can influence the availability of nutrients in fertigation systems. How do both of these parameters...

17 May 2021 1,495 4 View

How to upscale injection flow rate in the lab to field level?

I am trying to scale a reservoir fluid flow of 1-foot length per day in the reservoir to simulate the needed coreflood flow rate I should use in the lab. I have noticed that many coreflooding...

23 March 2020 4,399 6 View

In place of skim milk powder what can I use for preparing Skim Milk agar?

I'm about to prepare skim milk agar media to isolate protease producing bacteria but unfortunately I don't have Skim milk powder. I need answers to the best alternative to use as a substitute....

16 October 2019 8,517 3 View

Data formatting issues in HIERFSTAT?

I'm trying an analysis on HIERFSTAT for the first time using a subset of my data. I have some formatting issue that I can not put my finger on because when attempting to run basic.stats and other...

05 December 2018 10,166 2 View

Holotype or Lectotype?

Schlechter (1899) quoted two specimens when he described Mairia montana: Schlechter 9448 and 9751. Schlechter 9751 was later identified as Zyrphelis spathulata Zinnecker-Wiegand (Ortiz &...

05 April 2018 5,871 1 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View