Data formatting issues in HIERFSTAT?

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Geometrically Derived Formula for Day Length Based on Latitude and Time of Year?

Is there any geometrically derived mathematical expression for the duration of the natural day at any latitude and any time of the year?

23 June 2024 9,895 2 View

Is there a validated questionnaire about caregivers' health-care seeking behaviours for common childhood illnesses ?

Hello everyone, I hope everything is going well with you I'm looking for a validated questionnaire to use in a study to assess caregivers' health-care seeking behaviours for common childhood...

15 September 2023 4,560 0 View

Why there are a lot of colonies but no target fragment inserted?

I am going to insert a 6.3kb fragment into a 5.3kb plasmid. I use SAM1 digestion to cut the blunt end on the plasmid which is CCCGGG. I designed a set of primers to amplify the target fragment...

12 November 2022 7,351 3 View

How do I select substitution of codon without altering original function of the protein?

I want to mutate the ATGs of a gene, but I also want to not influence the original function of the protein. May I know whether there are criteria for selecting substitution of codon?

07 September 2022 1,718 1 View

Can mediation analysis be done only with indirect effect?

Hi, currently doing a mediation analysis for the following research framework (A -> mediated by C-> E) With such a framework, there's an indirect effect of mediation and no direct effect....

09 March 2022 3,672 3 View

How to successfully knockdown the SPP1 gene from MIA PACA 2 cells using siRNA?

I have been trying to transfect MIA PACA 2 using lipofectamine 2000. However, the real time PCR results (Ct values) are the same for the control and the experiment.

23 February 2022 687 3 View

How does water quality (pH and hardness of the water) effect the efficiency of foliar application of fertilizers?

pH can influence the efficiency for pesticides. We also know that hardness of the water can influence the availability of nutrients in fertigation systems. How do both of these parameters...

17 May 2021 1,494 4 View

How to mount a tissue sample of 1mm in thickness on a slide to do confocal microscope imaging?

I would like to analyze a 1mm in thickness skin sample using confocal microscopy. The sample is too thick to be placed between a slide and coverslip then sealed by nail polish. The idea is to do...

11 April 2021 3,114 4 View

How can I convert sodium hyaluronate to hyaluronic acid?

We sourced sodium hyaluronate that I need to convert to hyaluronic acid for drug delivery purposes. How can I do that? Would an ion-exchange resin do the job? Do you know of an established...

19 January 2021 8,769 0 View

How Can conduct measurement on powder using Raman Microscopy?

I am trying to measure the Raman microscopy spectrum of a drug in its powder form and I am not sure what is the best way to spread the powder over the microscope slide so that no interference will...

13 December 2020 8,223 6 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,099 4 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,428 2 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,402 2 View

What are the key methods and indicators used in assessing the biodiversity of river ecosystems, and how do these methods account for variations ?

Biodiversity assessment of river ecosystems is crucial for understanding the health and stability of these environments. This question aims to explore the various techniques employed to evaluate...

07 August 2024 4,289 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,105 4 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,472 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,129 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,703 7 View

Why 3 replicates for most biological assays? Is it enough to examine the data fits normal distribution?

Just bounced on me. Before statistically analysing significant difference, shouldn't we see if data fits normal distribution first? Is 3 replicates enough to testify the hypothesis of normal...

31 July 2024 8,141 13 View