Hi.
The purpose of this experiment is to know whether the bacteria exist in the bladder.
I extracted the DNA of rabbit's kidney and bladder using QIAGEN DNeasy Blood & Tissue kit.
And, I conducted 16s rRNA PCR using 16s primers 341F and 805R (used for 16S metagenomic sequencing library prep).
After running the gel electrophoresis, however, I found the band (about 1400-1500bp).
I think the the band can be the amplicon of animal tissue's DNA.
So, I want to know what the general (?) / sketchy (?) size of PCR product of the animal tissue when using those primers (341F / 805R or 27F / 1492R).
Thanks.
Hi Jang,
Normally if you use 16s primers, you will have bands with 1400- 1500 bp that is related to bacteria.
Mitochondria is the only things that can be detect by 16s primers in animal tissue, it has usually 500 bp band.
you can read more in the following paper:
Article Species identification through mitochondrial rRNA genetic analysis
Goog luck
Greetings,
Using the 16S Primers 341F and 806R will result in a product size of about ~440bp since the primers targets that "region of interest". But using 27F and 1492R will result in a product of ~1500bp.
Negar Bagheri Andrew Ting
Thanks for your answers.
I used only 341F, 805R primers, but I found the band (1400-1500 bp).
I think this band could be derived from the animal tissue.
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