Hello Azeri,

After co-cultive is essential that the cellular machinery assimilates the T-DNA region ( usually this region also contain the genes implicated in resistence to herbicides). This step must be maintained for 1-2 days, since usually this medium content acetosyringone as inductor of T-DNA transfer and insertion during Agrobacterium infection. One time the T-DNA region is inserted into genome, the plant can produced the genes implicated in resistence to herbicides and not before.

Good luck!

The main purpose of resting stage is to recover as many independent transgenic events as possible. Unless the multiple transgenic events would fuse together making it difficult to recover the events separately (Hiei and Komari, 2008).

the gene for resistance to the herbicide must be integrated in the genome of the cells and this gene must be replicated during the cell division like the other genes , at this moment that the cells acquire the resistance and this time we can add the herbicide and test the sensitivity of the plant material