Does anyone has a working protocol of sample preparation for doing NMR for G-quadruplex formation in presence of a specific Metal Ion ?

I want to do NMR for my G-quadruplex forming Oligonucleotides in presence of a specific metal ion. Our lab is new to this topic and no one in our lab has experienced about this.I have tried using...

17 March 2020 8,355 2 View

I need help to interpret my cox proportional model?

Hi There I was performing a cox proportional model for a cancer study in SPSS and I had this output where the Exp(B) was 0.0000064 and the CI 0 to 3.69E+51 ; p= 1 How would I report this Thank...

13 August 2019 5,054 3 View

How to create a bathymetry input file for MIKE 21?

Hi, I am learning MIKE 21 model and I would like to create input bathymetry file from GEBCO 30 Arc Second data for north coastal Bay of Bengal. Which one do I need to follow? Bathymetry or mesh...

24 June 2019 4,372 5 View

Why so called dark matter is outside the galaxy?

The so called dark matter is pictured out side the galaxy. What is the reason? why cant it be a part of the galaxy itself ?

03 September 2018 155 4 View

Will someone provide me typical sorts of laboratory exercises on Machine learning?

I want to develop a laboratory module for undergraduate computer science on Machine learning. The lab should consist of a few 15-20 questions ranging from simple ones to little complex ones...

05 August 2018 1,570 6 View

How to account for the time duration of polymer immersion in solvent, in the calculations for swelling ratio?

The Flory-Rehner equation does give the relation between cross-linking density and the swelling ratio. But will there arise a difference in the swelling ratio if a polymer is immersed in a solvent...

24 May 2018 9,437 3 View

Does Id/ Ig increase or decrease when GO goes to rGO?

I have been trying to understand this. A few works I came across seemed to have contradictory results. If the Id/ Ig does increase, when GO goes to rGO, does it mean that the removal of Oxygen...

22 May 2018 1,491 6 View

Experts please help to identify these plants?

Experts please help to identify these plants. images 1 and 2 are same plant.

17 December 2017 3,633 10 View

Experts please help me to identify this plant?

Dear experts please help me to identify this plant

02 August 2016 730 14 View

Where can I get Saw palmetto berries or plants in india?

11 January 2016 9,228 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View